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探究以多齿席夫碱配体H_2L与三(二苯甲酰甲烷)合铒(Ⅲ)二水合物[Er(dbm)3·2H_2O]制备的双核稀土配合物对尖孢镰刀菌的抑菌效果,为防治土传病害提供理论依据.采用溶剂热合成法制备新型双核稀土配合物,通过菌丝体生长率法和比浊法测定其对尖孢镰刀菌的抑制活性;运用紫外-可见分光光度滴定法、电化学循环伏安法及荧光淬灭法研究配合物与小牛胸腺DNA的相互作用机制.结果显示,浓度为160μg/mL、120μg/mL、60μg/mL的配合物在第5 d对尖孢镰刀菌的抑制率分别为77.2%、66.2%和46%,抑制率呈显著剂量依赖性递增(P<0.05),且相同浓度下抑菌率均高于配体及稀土盐;机制研究表明,配合物与DNA的结合方式主要为插入结合.该双核稀土配合物对尖孢镰刀菌具有优异的抑菌活性,其作用机制与DNA插入结合相关.本研究为新型抗菌金属配合物药物的设计与合成提供了重要参考.
Abstract：This study investigates the antibacterial effect of a binuclear rare earth complex prepared from the multidentate Schiff base ligand H_2L and tris(dibenzoylmethane)erbium(Ⅲ)dihydrate[Er(dbm)3·2H_2O]on Fusarium oxysporum,providing a theoretical basis for the prevention and control of soil borne diseases.A novel dual core rare earth complex was prepared by solvothermal synthesis method,and its inhibitory activity against Fusarium oxysporum was determined by mycelial growth rate method and turbidity method;The interaction mechanism between the complex and calf thymus DNA was studied using UV visible spectrophotometric titration,electrochemical cyclic voltammetry,and fluorescence quenching methods.The results showed that the complexes with concentrations of 160 μg/mL,120 μg/mL,and 60 μg/mL had inhibition rates of 77.2%,66.2%,and 46% against Fusarium oxysporum on the fifth day,respectively.The inhibition rates showed a significant dose-dependent increase(P<0.05),and were higher than those of ligands and rare earth salts at the same concentration;Mechanism studies have shown that the binding mode between the complex and DNA is mainly insertion binding.The dual core rare earth complex exhibits excellent antibacterial activity against Fusarium oxysporum,and its mechanism of action is related to DNA insertion binding.This study provides important references for the design and synthesis of novel antibacterial metal complex drugs.
[1]马艳，王光飞.生物炭防控植物土传病害研究进展[J].中国土壤与肥料，2014(6):14?20.
[2]马薇薇.分析作物土传病害的危害及防治技术[J].花卉，2020(8):263?264.
[3]DA ROSA?GARZON NG,LAURE H J,DE SOUZA?MOTTAC M,et al.Medium p H in submerged cultivation modulates differences in the intracellular protein profile of Fusarium oxysporum[J].Preparative Biochemistry and Biotechnology,2017,47(7):664?672.
[4]侯思远，林睿华，燕子红，等.稀土微肥在农业上的应用[J].化肥设计，2023,61(3):1?4,9.
[5]叶丽娟，杨德俊，李强国.稀土多元配合物的抑菌作用初步研究[J].湘南学院学报，2005,26(5):63?66.
[6]LI J L,ZHU Q Y,WU Y C,et al.Synthesis,characterization,and applications of rare?earth?based complexes with antibacterial and antialgal properties[J].ACS Applied Bio Materials,2024,7(1):104?113.
[7]石少明，覃江克，陈振锋，等.具有药用活性的稀土配合物在医药领域中的应用[J].华西药学杂志，2013,28(3):317?320.
[8]李金蕾.稀土基吡啶硫酮配合物的合成、结构及抗菌抗藻防污性能研究[D].赣州：江西理工大学，2023.
[9]梁玲玲，卢刚.哌嗪羧酸铜配合物的合成、表征及抑菌性能[J].化学与生物工程，2023,40(6):40?43.
[10]YANG L,WANG B C,TAN J,et al.The DNA?binding and bioactivity of rare earth metal complexes[J].Mini Reviews in Medicinal Chemistry,2013,13(10):1487?1500.
[11]欧阳泽英.含双苯并咪唑稀土金属配合物及其与DNA作用研究[D].长沙：湖南师范大学，2009.
[12]费丹，王兴明，阮开敏，等.茜素红S?钆（Ⅲ）配合物与DNA的相互作用[J].西南科技大学学报，2008,23(1):78?82,86.
[13]LUO J Y,WEI W L,WALDISPüHL J,et al.Challenges and current status of computational methods for docking small molecules to nucleic acids[J].European Journal of Medicinal Chemistry,2019,168:414?425.
[14]中华人民共和国国家市场监督管理总局，中国国家标准化管理委员会.微生物源抗生素类次生代谢产物抗真菌活性测定菌丝生长速率法：GB/T 38480?2020[S].北京：中国标准出版社，2020.
[15]周成勇.多胺类金属配合物的合成、表征及其与DNA作用的研究[D].太原：山西大学，2007.
[16]张靖雲，胡霞，姚琴，等.Sb3+与小牛胸腺DNA的相互作用及驱动机制[J].化学通报，2024,87(12):1428?1437.
[17]孙大猛.取代苯基三联吡啶钴、铁配合物的生物活性及与DNA/BSA结合的研究[D].南宁：广西大学，2024.
[18]NEBE T,XU J Y,BEITAT A,et al.Iron and cobalt complexes with the ligand(2?aminoethyl)bis(2?pyridylmethyl)amine(uns?penp)and derivatives[J].Inorganica Chimicaacta,2010,363(12):2965?2970.
[19]ZHANG X T,MA Z Y,ZHAO C,et al.Synthesis,crystal structures,DNA binding,and cytotoxicity activities of two copper(II)complexes based on unsymmetrical tripodal ligands[J].Journal of Coordination Chemistry,2015,68(13):2307?2323.
[20]赵晓飞，张培芳，曹冰雁，等.多吡啶镍配合物的合成表征及与DNA和HSA的作用[J].当代化工，2024,53(9):2156?2161.
[21]WANG L,LI W J,SONG Y M.Antitumor activity and DNA binding studies on rare earth metal complexes with all?trans retinoic acid and L?glutamic acid[J].RSC Advances,2014,4(80):42285?42292.
[22]GEHLEN M H.The centenary of the Stern?Volmer equation of fluorescence quenching:From the single line plot to the SVquenching map[J].Journal of Photochemistry and Photobiology C:Photochemistry Reviews,2020,42:100338.
基本信息:
中图分类号:O641.4;TQ450.1
引用信息:
[1]贺佳,燕平梅.稀土配合物对尖孢镰刀菌的抑制效果及其与DNA作用的探究[J].太原师范学院学报(自然科学版),2025,24(04):57-62.
基金信息:
土壤消毒、活化绿色产业技术创新战略联盟
2025-12-25
2025-12-25